SKU: 43028696991

Equine BETAEP (Beta-endorphin) ELISA Kit

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Description

Equine BETAEP (Beta-endorphin) ELISA KitThe Equine BETAEP (Beta endorphin) ELISA Kit measures Beta EP in Equine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with EP. EP in the sample or standard competes with a fixed amount of biotin labeled EP for sites on a pre coated antibody specific to EP. Free components are washed off. After adding

The Equine BETAEP (Beta-endorphin) ELISA Kit measures Beta-EP in Equine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with βEP. βEP in the sample or standard competes with a fixed amount of biotin-labeled βEP for sites on a pre-coated antibody specific to βEP. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of βEP in the sample. The concentration of the target substance is inversely proportional to the OD450 value.

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SKU: 43028696991

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